BIOIMPEDANCE PLATFORM TO ASSESS DRUGS AGAINST PHOTO-OXIDATIVE CELL BEHAVIOR USING HDFN CELLS
Published: 2 Jun 2014
Abstract: Light radiation plays an important role in skin diseases. In thisstudy, wehaveprovideda simple,high-throughput and real-time bio-analytical method to measure the radiation effect on the human dermal fibroblastneonatal (HDFn) cells by electric cell substrate impedance sensing (ECIS) system. Thisbio-analytical system was utilized for radiation effects on cells that exposed to various bio-active compounds to treatdamage cells. The ECIS physical response is well correlated with standard qualitative results. Prolonged light-exposure caused time dependent growth drop in HDFn cells and these resulted in photo-oxidative damage due to deregulation of intra and extra cellular biochemical activities followed by cell cycle arrest. ECIS measurements show< 50mMconcentrationswere found to bewell tolerated non-toxic safer and its show good protection against light radiation toxicity. The proposed system guides the effective in-vitro model, and would be of wide interest in the field of cosmetics and therapeutics.
Keywords: electric cell-substrate impedance sensing (ecis), photo-protective drugs, light radiation induced damage, cell behaviour, in-vitro light setup
Download full text
Back to the contents of the volume
© 2017 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/
, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This permission does not cover any third party copyrighted material which may appear in the work requested.