BIOIMPEDANCE PLATFORM TO ASSESS DRUGS AGAINST PHOTO-OXIDATIVE CELL BEHAVIOR USING HDFN CELLS
Published: 2 Jun 2014
Abstract: Light radiation plays an important role in skin diseases. In thisstudy, wehaveprovideda simple,high-throughput and real-time bio-analytical method to measure the radiation effect on the human dermal fibroblastneonatal (HDFn) cells by electric cell substrate impedance sensing (ECIS) system. Thisbio-analytical system was utilized for radiation effects on cells that exposed to various bio-active compounds to treatdamage cells. The ECIS physical response is well correlated with standard qualitative results. Prolonged light-exposure caused time dependent growth drop in HDFn cells and these resulted in photo-oxidative damage due to deregulation of intra and extra cellular biochemical activities followed by cell cycle arrest. ECIS measurements show< 50mMconcentrationswere found to bewell tolerated non-toxic safer and its show good protection against light radiation toxicity. The proposed system guides the effective in-vitro model, and would be of wide interest in the field of cosmetics and therapeutics.
Keywords: electric cell-substrate impedance sensing (ecis), photo-protective drugs, light radiation induced damage, cell behaviour, in-vitro light setup
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