BIOIMPEDANCE PLATFORM TO ASSESS DRUGS AGAINST PHOTO-OXIDATIVE CELL BEHAVIOR USING HDFN CELLS
Devasier Bennet, Sakthivel Ramasamy, Jeong Ho An, Dong Jun Chung, Sanghyo Kim
Pages: 659-666 Published: 2 Jun 2014
Views: 2,183 Downloads: 1,244
Abstract: Light radiation plays an important role in skin diseases. In thisstudy, wehaveprovideda simple,high-throughput and real-time bio-analytical method to measure the radiation effect on the human dermal fibroblastneonatal (HDFn) cells by electric cell substrate impedance sensing (ECIS) system. Thisbio-analytical system was utilized for radiation effects on cells that exposed to various bio-active compounds to treatdamage cells. The ECIS physical response is well correlated with standard qualitative results. Prolonged light-exposure caused time dependent growth drop in HDFn cells and these resulted in photo-oxidative damage due to deregulation of intra and extra cellular biochemical activities followed by cell cycle arrest. ECIS measurements show< 50mMconcentrationswere found to bewell tolerated non-toxic safer and its show good protection against light radiation toxicity. The proposed system guides the effective in-vitro model, and would be of wide interest in the field of cosmetics and therapeutics.