AMPEROMETRIC BIOSENSOR FOR DETERMINATION OF TOTAL TEA-CATECHINS CONTENT IN COMMERCIAL KOREAN AND JAPANESE TEAS
Published: 25 Sep 2014
Abstract: The reductive detection of H2O2 with a membrane-covered horseradish peroxidase (HRP)-entrapped, ferrocene (Fc)-embedded carbon paste (CP) electrode is applied to determine the total amount of polyphenols in commercial teas. The steady-state reduction current of H2O2 decreases with the addition of polyphenol into a buffer solution containing H2O2 and HRP. The magnitude of the decreased current is proportional to the amount of the added polyphenol and the sensitivity of the detection limit depends on the electron-donating properties of polyphenol. For (+)-catechin, the HRP-Fc-CP electrode allows the detection limit to come down to 0.3 μM with a linear relation going up to 150 μM and without changing of the response over one month. The total amounts of polyphenols in several teas detected by the present method are well correlated to those determined by the photometric determination.
Keywords: amperometric biosensor, hrp, carbon paste electrode, commercial teas, catechins
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