1,2-DIBROMOETHANE BIODEGRADATION CAPACITY OF BRADYRHIZOBIUM JAPONICUM STRAIN 273
Published: 27 Jul 2018
Abstract: 1,2-Dibromoethane is an extremely toxic chemical owing to its cancer-causing potential. Many years after its last known application as a soil fumigant, residual 1,2-DBE is still found at remarkably high concentrations in soil because it strongly interacts with the soil matrix. Its biodegradation passes through very toxic intermediates e.g. bromoethanol and bromo-acetaldehyde and that is why the complete biodegradation is not observed at higher substrate concentrations. This work examines potentials of Bradyrhizobium japonicum 273 strain for degrading 1,2-DBE.. The experiments were carried out in shaking flasks with various initial concentration of substrate: 0,05 g/l; 0,1 g/l; 0,2 g/l and 0,3 g\l 1,2-DBE. In order to verify biodegradation, formation of biodegradation products like bromoethanol and bromide ions were measured. The concentration of biomass, generated bromoethanol and the quantity of bromide ions were determined spectrophotometrically, and were calculated from the optical density, using a calibration curve. Complete mineralization was verified by measuring stoichiometric release of the biodegradation products.
Keywords: 1,2-dibromoethane, bradyrhizobium japonicum 273, bromoethanol, bromide ions
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