EPIDEMIOLOGIC APPROACHES OF BRUCELLOSIS THROUGH PHYLOGENETIC ANALYSIS PATTERN IN PALESTINE
Elena Awwad, Stelian Baraitareanu, Maria Rodica Gurau, Mohammad Faraj, Tamer Essawi, Kamel Adwan, Doina Danes
Strony: 267-275
Opublikowano: 6 Jun 2016
Wyświetlenia: 3,031
Pobrania: 525
Citations: 2 (Google Scholar)
Streszczenie: Brucellosis is an endemic zoonotic disease of the small ruminants in Palestine. The previous study revealed that the lpsB gene (responsible for the biosynthesis of mannosyltransferase) of Brucella melitensis has a degree of genetic diversity. Despite these variations, the bacteria keep its virulence and ability to produce abortion and human infection. The aim of this study was to identify the evolutionary relationships between 18 field strains of Brucella melitensis isolated from herds from West Bank, and to compare them with strains from different countries, using phylogenetic analysis, in order to characterize their epidemiological pattern. The sequencing of the lpsB gene of the 18 field strains has been performed with ABI PRISM 3500 Genetic Analyzer. The alignment and the phylogenetic analysis between field isolates, Brucella melitensis reference strain and Rev 1 strains were done with CLC Workbench version 6.1.5 software. The analysis revealed that all field isolates were closely related to each other: isolates from neighboring districts have had close variations, but the strongest relationship was among strains from flocks of the same village. The isolates from some flocks were closer to Brucella melitensis Rev 1, suggesting the exposure or infection of these with the vaccinal strain. Viewed in a global context, the West Bank isolates were phylogenetically closest to the Spanish isolates. This study highlights the input of the phylogenetic tree analysis as an epidemiological tool for the investigation of Brucella infection.
Słowa kluczowe: brucellosis, zoonosis, phylogenetic tree, alignment
Cytowanie artykułu: Elena Awwad, Stelian Baraitareanu, Maria Rodica Gurau, Mohammad Faraj, Tamer Essawi, Kamel Adwan, Doina Danes. EPIDEMIOLOGIC APPROACHES OF BRUCELLOSIS THROUGH PHYLOGENETIC ANALYSIS PATTERN IN PALESTINE. Journal of International Scientific Publications: Agriculture & Food 4, 267-275 (2016). https://www.scientific-publications.net/en/article/1001041/
Powrót do spisu treści tomu
© 2026 The Author(s). This is an open access article distributed under the terms of the
Creative Commons Attribution License https://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This permission does not cover any third party copyrighted material which may appear in the work requested.
Disclaimer: The Publisher and/or the editor(s) are not responsible for the statements, opinions, and data contained in any published works. These are solely the views of the individual author(s) and contributor(s). The Publisher and/or the editor(s) disclaim any liability for injury to individuals or property arising from the ideas, methods, instructions, or products mentioned in the content.
