INFLUENCE OF FE3O4 /OLEIC ACID - CORE/SHELL NANOPARTICLES ON THE CELLULAR CYCLE AND ADHERENCE OF DIFFERENT EUKARYOTIC CELL LINES
Alexandru Mihai Grumezescu, Coralia Bleotu, Crina Saviuc, Carmen Chifiriuc, Dan Mihaiescu, Alina Holban, Ani Cotar, Marcela Bucur, Luminita Dascalu, Veronica Lazar
Pagini: 48-53
Publicat: 1 Jan 2011
Vizualizări: 79
Rezumat: The purpose of the present work was to investigate the influence of Fe3O4/oleic acid - core/shell nanoparticles on the cellular cycle, adherence and monolayer development of different eukaryotic cell lines. Core/shell nanoparticles have been synthesized by Massart adapted method, under microwave conditions. The dimensions of Fe3O4/oleic acid nanoparticles were in the 5-20 nm range and they were previously characterized by High Resolution Transmission Electron Microscopy. The nanoparticles were deposed on glass slides in one, two and respectively three layers, and thereafter introduced in 6-multi well plastic plates and sterilized by ethanol 70%, for 10 min, followed by UV treatment. The Human epithelial carcinoma (HeLa) and the Human colonic epithelial cancer (HCT) o cell lines were cultivated on the pelliculised glass slides and incubated for 24 hours at 37 C in 5% CO2 atmosphere. Thereafter, the slides were fixed by ethanol, Giemsa stained and examined in 5 reversed microsocopy. For the assessment of the cellular cycle, 3x10 eukarotic cells were put in Petri dishes and treated for 24 hours with nanoparticles. The cells were further fixed by ethanol, stained with propidium iodide and quantified by flow cytometry (Facscalibur). Our results showed that the magnetic nanoparticles of Fe3O4 / oleic acid - core/shell exhibit specific influences on the ability of different cell lines to colonize the glass substrata.
Cuvinte cheie: nanoparticles, core/shell, hct, hela, hr-tem, cell line
Citează acest articol: Alexandru Mihai Grumezescu, Coralia Bleotu, Crina Saviuc, Carmen Chifiriuc, Dan Mihaiescu, Alina Holban, Ani Cotar, Marcela Bucur, Luminita Dascalu, Veronica Lazar. INFLUENCE OF FE3O4 /OLEIC ACID - CORE/SHELL NANOPARTICLES ON THE CELLULAR CYCLE AND ADHERENCE OF DIFFERENT EUKARYOTIC CELL LINES. Journal of International Scientific Publications: Materials, Methods & Technologies 5, 48-53 (2011). https://www.scientific-publications.net/en/article/1003285/
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