International Scientific Publications
© 2007-2025 Science Events Ltd
Terms of Use  ·  Privacy Policy
Language English French Polish Romanian Bulgarian
Conference room
Agriculture & Food 2025, 13th International Conference
11-14 August, Burgas, Bulgaria
Call for Papers

Agriculture & Food, Volume 2, 2014

IMPROVED ANALYTICAL METHOD OF SYNTHETIC FOOD COLOUR ADDITIVE, BROWN HT BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Mi-Na Hong, Hee-Jae Suh, Ok-Hwan Lee, Hyang-Sook Chun, Chan Lee
Pages: 68-75
Published: 1 Jun 2014
Views: 4,322
Downloads: 1,950
Abstract: Brown HT is bis-azo dye which is permitted in EU as a color additive. C18 column was generally applied in analysis of Brown HT in HPLC system using diode array detection with sodium sulfate or phosphate solution as an eluent. C4 column can also be used with 100% methanol as mobile phase. Low recovery, reproducibility, and linearity are the major limitations in previous reported methods. The purpose of this study is to optimize the method using different solvents. In our study, the best resolution of Brown HT analysis was observed with following eluent; solvent A of mobile phase was 0.575g mono ammonium phosphate, 0.7g disodium hydrogen phosphate in 500mL water added with 500mL methanol (pH 6.9) and solvent B was methanol. This method exhibited quite high recovery and reproducibility of Brown HT and it presented better result compared to the previous studies. LC-MS/MS was also used to confirm Brown HT.
Keywords: colour additive, food additive, high-performance liquid chromatography (hplc), lc-ms/ms, brown ht, ci food brown 3, e155
Cite this article: Mi-Na Hong, Hee-Jae Suh, Ok-Hwan Lee, Hyang-Sook Chun, Chan Lee. IMPROVED ANALYTICAL METHOD OF SYNTHETIC FOOD COLOUR ADDITIVE, BROWN HT BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY. Journal of International Scientific Publications: Agriculture & Food 2, 68-75 (2014). https://www.scientific-publications.net/en/article/1000010/
Back to the contents of the volume

Submit Feedback

We value your input! Use this form to report any concerns or provide feedback on our published articles. All submissions will be kept confidential.

By using this site you agree to our Privacy Policy and Terms of Use. We use cookies, including for analytics, personalisation, and ads.