IONIC LIQUIDS – BASED BIPHASIC SYSTEMS FOR ENZYME EXTRACTION: PRELIMINARY DATA FROM IONIC LIQUIDS’ SCREENING
Konstantza Tonova, Ivan Svinyarov, Milen Bogdanov
Pages: 442-451 Published: 31 May 2015
Views: 1,900 Downloads: 626
Abstract: Ionic liquids consisting of pairs of imidazolium or phosphonium cation and saccharinate or chloride anion were synthesized and examined for extraction efficiency towards the hydrolytic enzyme, α-amylase. The quantification of the enzyme partitioning in the equilibrated biphasic systems of ionic liquid and water was based on the α-amylase activity assay, performed where possible in both phases. The water transfer from the aqueous phase into the ionic liquid-rich phase was also followed. The effect of pH, which determines the electrostatic charge of the protein aminoacid chain, was studied. Backward extractions were also performed in order to evaluate the activity yield resulted from the enzyme transfer though the ionic liquid.