International Scientific Publications
© 2018 Science Events Ltd
Terms of Use  ·  Privacy Policy
Choose language English French Russian Bulgarian

Agriculture & Food, Volume 4, 2016

COMPARISON OF MOLECULAR ASSAYS FOR THE DETECTION OF ALLEXIVIRUSES IN GARLIC PLANTS
Bereda M., Paduch-Cichal E., Kalinowska E., Szyndel M. S.
Pages: 67-73
Published: 6 Jun 2016
Views: 934
Downloads: 323
Abstract: Allexiviruses and several Carla- and Potyvirus species are the most widespread viral pathogens of Allium crops world-wide. Simultaneous infection by these viruses causes significant reduction of bulb weight. Production of species-specific antisera against allexiviruses is complicated. Production of virus-free propagation material requires the use of highly specific, sensitive and rapid virus detection methods. The aim of the present study therefore was to examine primer sequences using RT-PCR and IC-RT-PCR method and testing the dot-blot hybridization for detection of allexiviruses. Electron microscope was also used for selective garlic plants infected with Allexiviruses.
Keywords: allexivirus, rt-pcr, ic-rt-pcr, dot-blot hybridization
Download full text

Back to the contents of the volume
By using this site you agree to our Privacy Policy and Terms of Use. We use cookies, including for analytics, personalisation, and ads.