PROTEIN FRACTION ANALYSIS OF MILK PRODUCED BY TWO GENETIC TYPES OF RABBITS: SYNTHETIC LINE AND WHITE POPULATION, RAISED IN THE REGION OF TIZI OUZOU, ALGERIA
Published: 6 Jun 2016
Abstract: The objective of the study was to analyze milk protein composition (major proteins) of two genetic types of rabbits bred in Tizi-Ouzou (Algeria): the white population (PB) and the "synthetic" line (SS). Differences in size and litter weight at weaning were observed amongst the two types, which might be due to qualitative and/or quantitative variations in milk composition. Milk samples (n=7) from dams of the two genetic types were collected (10th of lactation) and analyzed by means of liquid chromatography associated to a mass spectrometer (LC-MS). We were thus able to identify and characterize the major milk proteins in both types of rabbits and determine their post-translational modification isoforms. Alpha-s1 and β represented 50% of the total milk proteins; αs2-like-, αs2- and κ- caseins represented respectively 13.5, 4 and 2.7%. Whey Acidic Protein was the main whey protein (14.5%) while lactoferrin represented 10% of the total milk proteins. Chromatographic profiles of milk proteins were similar between the two genetic types of rabbits. In the "synthetic" strain, a strong dispersal around the mean of the relative proportions of αs2 casein and the mix α-Lac + SA was observed (CVαs2 = 0.24 in PB vs. 0.46 in SS and CVα-Lac + SA = 0.19 vs. 0.274 in PB and in SS respectively).
Keywords: lc-ms, milk, rabbit, protein
Download full text
Back to the contents of the volume
© 2018 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/
, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This permission does not cover any third party copyrighted material which may appear in the work requested.