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Agriculture & Food, Volume 4, 2016

PROTEIN FRACTION ANALYSIS OF MILK PRODUCED BY TWO GENETIC TYPES OF RABBITS: SYNTHETIC LINE AND WHITE POPULATION, RAISED IN THE REGION OF TIZI OUZOU, ALGERIA
Amroun T. T., Bianchi L., Zerrouki-Daoudi N., Lebas F., Charlier M., Devinoy E., Martin P., Miranda G.
Pages: 359-365
Published: 6 Jun 2016
Views: 834
Downloads: 303
Abstract: The objective of the study was to analyze milk protein composition (major proteins) of two genetic types of rabbits bred in Tizi-Ouzou (Algeria): the white population (PB) and the "synthetic" line (SS). Differences in size and litter weight at weaning were observed amongst the two types, which might be due to qualitative and/or quantitative variations in milk composition. Milk samples (n=7) from dams of the two genetic types were collected (10th of lactation) and analyzed by means of liquid chromatography associated to a mass spectrometer (LC-MS). We were thus able to identify and characterize the major milk proteins in both types of rabbits and determine their post-translational modification isoforms. Alpha-s1 and β represented 50% of the total milk proteins; αs2-like-, αs2- and κ- caseins represented respectively 13.5, 4 and 2.7%. Whey Acidic Protein was the main whey protein (14.5%) while lactoferrin represented 10% of the total milk proteins. Chromatographic profiles of milk proteins were similar between the two genetic types of rabbits. In the "synthetic" strain, a strong dispersal around the mean of the relative proportions of αs2 casein and the mix α-Lac + SA was observed (CVαs2 = 0.24 in PB vs. 0.46 in SS and CVα-Lac + SA = 0.19 vs. 0.274 in PB and in SS respectively).
Keywords: lc-ms, milk, rabbit, protein
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