CHOLINESTERASE INHIBITOR BIOSENSORS
Published: 1 Jun 2017
Abstract: This paper describes the partial results of the development of a new biosensor of cholinesterase inhibitors based on biochemical enzymatic reaction with colorimetric evaluation. A biosensor consists of an indicator zone with immobilised enzyme (butyrylcholinesterase), an auxiliary zone with a substrate (butyrylthiocholine) and a chromogenic agent, as well as a zone with colour etalon. The presence of cholinesterase inhibitors is indicated by the fact that there is no discoloration of the chromogenic reagent. A biosensor is suitable for the rapid and simple detection of chemical warfare agents (nerve gases) or industrial pesticides (organophosphates, carbamates) in water and in the air.
Keywords: butyrylcholinesterase, 2,6-dichlorophenolindophenol, physostigmine, inhibitors
Cite this article: Vladimír Pitschmann, Lukáš Matějovský, Michal Dymák, Tomáš Dropa, Martin Urban, Iva Vošahlíková. CHOLINESTERASE INHIBITOR BIOSENSORS. Journal of International Scientific Publications: Ecology & Safety 11, 18-23 (2017). https://www.scientific-publications.net/en/article/1001349/
Download full text
Back to the contents of the volume
© 2023 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/
, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This permission does not cover any third party copyrighted material which may appear in the work requested.