International Scientific Publications
© 2018 Science Events Ltd
Terms of Use  ·  Privacy Policy
Choose language English French Russian Bulgarian

Agriculture & Food, Volume 5, 2017

GENERATION OF TRANSGENIC CUCUMBER WITH RESISTANCE TO CUCURBIT CHLOROTIC YELLOWS VIRUS USING POLLEN ELECTROTRANSFORMATION
Hsiu-Fung Chao, Yi-Fang Hsu, Yung-Fu Yen
Pages: 16-25
Published: 17 Jul 2017
Views: 517
Downloads: 236
Abstract: The Cucurbit chlorotic yellows virus (CCYV) infects many Cucurbitaceae crops, resulting in chlorotic leaves, retarded growth, and a decreased yield. Transformed cucumber plants incorporating the coat protein (CP) gene of the CCYV were generated using the transformant of CP/pCAMBIA1302 vector. The plasmid DNA was introduced into the pollen through electroporation at voltage intensity of 25 μF × 1.5kV. The electropulsed pollen was used to pollinate emasculated flowers. The transformed T1 offspring were verified using PCR and about 22% of the T1 resistant offspring exhibited CP protein expression, as verified by Western blot analysis of the GFP. In the field evaluation, the transformed plants of the T3 generation showed a high level of resistance to the CCYV. Our results showed that these transgenic cucumber plants were resistance against the CCYV, which they obtained by incorporating the CP gene through a pollen electrotransformation approach.
Keywords: cucurbit chlorotic yellows virus, pollen electrotransformation, transgenic cucumber, coat protein
Download full text

Back to the contents of the volume
By using this site you agree to our Privacy Policy and Terms of Use. We use cookies, including for analytics, personalisation, and ads.