GENERATION OF TRANSGENIC CUCUMBER WITH RESISTANCE TO CUCURBIT CHLOROTIC YELLOWS VIRUS USING POLLEN ELECTROTRANSFORMATION
Published: 17 Jul 2017
Abstract: The Cucurbit chlorotic yellows virus (CCYV) infects many Cucurbitaceae crops, resulting in chlorotic leaves, retarded growth, and a decreased yield. Transformed cucumber plants incorporating the coat protein (CP) gene of the CCYV were generated using the transformant of CP/pCAMBIA1302 vector. The plasmid DNA was introduced into the pollen through electroporation at voltage intensity of 25 μF × 1.5kV. The electropulsed pollen was used to pollinate emasculated flowers. The transformed T1 offspring were verified using PCR and about 22% of the T1 resistant offspring exhibited CP protein expression, as verified by Western blot analysis of the GFP. In the field evaluation, the transformed plants of the T3 generation showed a high level of resistance to the CCYV. Our results showed that these transgenic cucumber plants were resistance against the CCYV, which they obtained by incorporating the CP gene through a pollen electrotransformation approach.
Keywords: cucurbit chlorotic yellows virus, pollen electrotransformation, transgenic cucumber, coat protein
Cite this article: Hsiu-Fung Chao, Yi-Fang Hsu, Yung-Fu Yen. GENERATION OF TRANSGENIC CUCUMBER WITH RESISTANCE TO CUCURBIT CHLOROTIC YELLOWS VIRUS USING POLLEN ELECTROTRANSFORMATION. Journal of International Scientific Publications: Agriculture & Food 5, 16-25 (2017). https://www.scientific-publications.net/en/article/1001389/
Download full text
Back to the contents of the volume
© 2023 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/
, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This permission does not cover any third party copyrighted material which may appear in the work requested.