HPLC-DAD METHOD FOR ANALYSIS OF GLYCOALKALOIDS ALPHA-SOLANINE AND ALPHA CHACONINE IN CONVENTIONAL POTATO
Irena Petrova, Violeta Ivanova-Petropolus, Liljana Koleva-Gudeva
Pages: 312-320
Published: 13 Nov 2023
DOI: 10.62991/AF1996325925
Views: 247
Downloads: 30
Abstract: A simple and fast HPLC-DAD method has been developed for separation and quantification of two glycoalkaloids, alpha-solanine and alpha-chaconine, in potatoes. Separation of the analytes was performed on a Shimadzu Shim-pack GIST C18 column (250mm x 4mm I.D., 5μm particle size) applying isocratic elution of the mobile phase consisting of 30% (v/v) acetonitrile and 70 % KH2PO4 (20 mM and pH 6.57). The flow rate on the mobile phase was 1 mL/min and the detection of solanine and chaconine was performed on a wavelength of 204 nm. The retention time of α-solanine was 12.8 min and for α-chaconine 14.6 min. Liquid-liquid extraction of analytes was performed from dehydrated potatoes using methanol-acetic acid (95:5, v/v), followed by SPE with Sep-Pak C18 columns. Satisfactory results for the repeatability and accuracy of the method confirmed that the extraction method as well as HPLC method are appropriate for analysis of alpha-solanine and alpha- chaconine in potatoes samples.
Keywords: alpha-solanine,alpha-chaconine, potatoes. hplc-dad
Cite this article: Irena Petrova, Violeta Ivanova-Petropolus, Liljana Koleva-Gudeva. HPLC-DAD METHOD FOR ANALYSIS OF GLYCOALKALOIDS ALPHA-SOLANINE AND ALPHA CHACONINE IN CONVENTIONAL POTATO. Journal of International Scientific Publications: Agriculture & Food 11, 312-320 (2023). https://doi.org/10.62991/AF1996325925
Download full text
Back to the contents of the volume
© 2024 The Author(s). This is an open access article distributed under the terms of the
Creative Commons Attribution License https://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This permission does not cover any third party copyrighted material which may appear in the work requested.
Disclaimer: The opinions and claims presented in this article are solely those of the authors and do not necessarily reflect the views of their affiliated organizations, the publisher, editors, or reviewers.
