THERMAL AND MECHANICAL DENATURATION OF CELLULASE IN SOLUTION AND IN THE SOLID PHASE
Aleksey L. Bychkov, Vladimir A. Buchtoyarov, Oleg I. Lomovsky
Pages: 4-10
Published: 1 Jan 2011
Views: 127
Abstract: Decrease of cellulosolytic activity Trichoderma viride cellulase under heating and mechanical treatment in solution and in solid phase is investigated. A comparison between the stabilities of the enzymatic preparation to thermal denaturation in the dry and dissolved formed is carried out. Kinetic parameters characterizing denaturation process are determined. The solutions of enzymes are stable at temperatures below 55 оC and rapidly get denaturated at a temperature above 65 оC. Under heating in the solid phase, the enzymes exhibit higher stability and get denaturated rapidly only at temperatures above 100 оC. Rapid denaturation of the enzymatic preparation in the solid phase is observed during mechanical treatment with the intensity more than 1.25 W/g. The rate of mechanical denaturation is weakly dependent on the process temperature.
Keywords: enzymes, thermal inactivation, mechanical inactivation, stability, mechanoenzymatic processes
Cite this article: Aleksey L. Bychkov, Vladimir A. Buchtoyarov, Oleg I. Lomovsky. THERMAL AND MECHANICAL DENATURATION OF CELLULASE IN SOLUTION AND IN THE SOLID PHASE. Journal of International Scientific Publications: Materials, Methods & Technologies 5, 4-10 (2011). https://www.scientific-publications.net/en/article/1003280/
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