MICROWAVE-ASSISTED EXTRACTION OF FLAVONOID ANTIOXIDANTS FROM LEAVES OFHABERLEA RHODOPENSIS
Dasha Sp. Mihaylova, Slava T. Bahchevanska, Valentina T. Toneva
Pages: 104-114
Published: 1 Jan 2011
Views: 107
Abstract: Haberlea rhodopensis (Gesneriaceae) is a less explored endemic plant within a very small group of poikilohydric angiosperms that are able to survive in an almost completely dehydrated state. The purpose of the present study was to obtain extracts from Haberlea rhodopensis leaves using alternative technique- microwave-assisted extraction and to determine the total phenolic content and antioxidant activity of the extract by applying reliable methods: reducing power and ABTS radical cation decolorization assay. Different extraction conditions- microwave power and extraction times were investigated. Total phenolic content was determined using a spectrophotometric technique based on the Folin-Ciocalteu reagent and calculated as mgGAE/gDW (leaf dry weight). The results from the total phenolic assay and the antioxidant activity tests were significantly correlated. Major phenolic acids and some flavonoid- aglycones and - glycosides were analyzed by HPLC. Luteolin (3307.08 μg/gDW), myricetin (702.0 μg/gDW), ferulic acid (652.92 μg/gDW) and hesperidin (607.32 μg/gDW) were predominant in the H. rhodopensis microwave-assisted extracts and some of the effects of the plant could be attributed to the presence of these valuable constituents.
Keywords: haberlea rhodopensis, microwave extraction, antioxidant activity, hplc
Cite this article: Dasha Sp. Mihaylova, Slava T. Bahchevanska, Valentina T. Toneva. MICROWAVE-ASSISTED EXTRACTION OF FLAVONOID ANTIOXIDANTS FROM LEAVES OFHABERLEA RHODOPENSIS. Journal of International Scientific Publications: Materials, Methods & Technologies 5, 104-114 (2011). https://www.scientific-publications.net/en/article/1003289/
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