MONITORING OF MICROBIAL ADHESION AND BIOFILM GROWTH USING ELECTROCHEMICAL IMPEDANCEMETRY
Martin Martinov, Andre Darchen, Dimitre Hadjiev
Pagini: 334-346
Publicat: 1 Jan 2011
Vizualizări: 90
Rezumat: The possibility to use electrochemical impedance spectroscopy to monitor the cell attachment and the biofilm proliferation was tested. In order to identify characteristic events induced on the metal surface Gram-negative (Pseudomonas aeruginosa PAO1) and Gram-positive (Bacillus subtillis) bacteria strains have been studied. Electrochemical impedance spectra of AISI 304 electrodes during cell attachment and initial biofilm growth for both strains were obtained. It can be observed that the resistance increases gradually with the culture time and decreases with the biofilm detachment. So, the applicability of electric cell-substrate impedance sensing (ECIS) for studying the attachment and spreading of cells on a metal surface has been demonstrated. The biofilm formation was also characterized by the use of SEM and confocal laser scanning microscopy and COMSTAT image analysis. The electrochemical results roughly agree with the microscope image observations. The obtained results have been compared to existing data and the possibility to use the method for biofilm characterization was discussed. The ECIS technique used in this study was used for continuous real- time monitoring of the initial bacterial adhesion and the biofilm growth. It provides a simple and non expensive electrochemical method for in vitro assessment of the presence of biofilms on metal surfaces.
Cuvinte cheie: electrochemical impedance, microbial adhesion, biofilm thickness
Citează acest articol: Martin Martinov, Andre Darchen, Dimitre Hadjiev. MONITORING OF MICROBIAL ADHESION AND BIOFILM GROWTH USING ELECTROCHEMICAL IMPEDANCEMETRY. Journal of International Scientific Publications: Materials, Methods & Technologies 5, 334-346 (2011). https://www.scientific-publications.net/en/article/1003311/
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