HPLC-DAD METHOD FOR ANALYSIS OF GLYCOALKALOIDS ALPHA-SOLANINE AND ALPHA CHACONINE IN CONVENTIONAL POTATO
Irena Petrova, Violeta Ivanova-Petropolus, Liljana Koleva-Gudeva
Страницы: 312-320
Опубликовано: 13 Nov 2023
DOI: 10.62991/AF1996325925
Просмотры: 2,377
Загрузки: 228
Captures: 2 (Elsevier PlumX)
Аннотация: A simple and fast HPLC-DAD method has been developed for separation and quantification of two glycoalkaloids, alpha-solanine and alpha-chaconine, in potatoes. Separation of the analytes was performed on a Shimadzu Shim-pack GIST C18 column (250mm x 4mm I.D., 5μm particle size) applying isocratic elution of the mobile phase consisting of 30% (v/v) acetonitrile and 70 % KH2PO4 (20 mM and pH 6.57). The flow rate on the mobile phase was 1 mL/min and the detection of solanine and chaconine was performed on a wavelength of 204 nm. The retention time of α-solanine was 12.8 min and for α-chaconine 14.6 min. Liquid-liquid extraction of analytes was performed from dehydrated potatoes using methanol-acetic acid (95:5, v/v), followed by SPE with Sep-Pak C18 columns. Satisfactory results for the repeatability and accuracy of the method confirmed that the extraction method as well as HPLC method are appropriate for analysis of alpha-solanine and alpha- chaconine in potatoes samples.
Ключевые слова: alpha-solanine,alpha-chaconine, potatoes. hplc-dad
Цитировать эту статью: Irena Petrova, Violeta Ivanova-Petropolus, Liljana Koleva-Gudeva. HPLC-DAD METHOD FOR ANALYSIS OF GLYCOALKALOIDS ALPHA-SOLANINE AND ALPHA CHACONINE IN CONVENTIONAL POTATO. Journal of International Scientific Publications: Agriculture & Food 11, 312-320 (2023). https://doi.org/10.62991/AF1996325925
Вернуться к содержанию тома
© 2026 The Author(s). This is an open access article distributed under the terms of the
Creative Commons Attribution License https://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This permission does not cover any third party copyrighted material which may appear in the work requested.
Disclaimer: The Publisher and/or the editor(s) are not responsible for the statements, opinions, and data contained in any published works. These are solely the views of the individual author(s) and contributor(s). The Publisher and/or the editor(s) disclaim any liability for injury to individuals or property arising from the ideas, methods, instructions, or products mentioned in the content.
