APPLICATION OF BIOLUMINESCENT ENZYMATIC TESTS IN ECOTOXICOLOGY
Published: 27 Jul 2018
Abstract: This paper examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent Enzyme System Technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH:FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For wide-spread use of BEST, the multi-component reagent ‘Enzymolum’ has been developed, which contains the bacterial luciferase, NADH:FMN-oxidoreductase and their substrates, co-immobilized in starch or gelatin gel. ‘Enzymolum’ can be integrated as a biological module into the portable biodetector-biosensor originally constructed for personal use. Based on the example of ‘Enzymolum’ and the algorithm of creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 minutes), high sensitivity, simplicity and safety of procedure, possibility of automation of ecological monitoring; required luminometer is easily available.
Keywords: bioluminescence, luciferase, enzymatic assay, immobilization, integral water toxicity, ecological monitoring
Cite this article: Elena N. Esimbekova, Valeriya P. Kalyabina, Valentina A. Kratasyuk. APPLICATION OF BIOLUMINESCENT ENZYMATIC TESTS IN ECOTOXICOLOGY. Journal of International Scientific Publications: Ecology & Safety 12, 135-146 (2018). https://www.scientific-publications.net/en/article/1001655/
Download full text
Back to the contents of the volume
© 2021 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/
, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This permission does not cover any third party copyrighted material which may appear in the work requested.