Materials, Methods & Technologies, Volume 13, 2019

Abstract: The compounds as 1, 2-dibromoethane, 1, 2-dichloroethane and phenol are ones of the most dangerous pollutants in the environment. 1, 2-Dibromoethane (DBE) is a synthetic organic chemical that is mainly used as a gasoline additive. It is also one of the widely used pesticide fumigants. 1,2-Dichloroethane is one of the most commonly used chlorinated industrial products and falls into the environment by using it as a chemical intermediate in the synthesis of a number of chlorinated hydrocarbons. Phenol is a waste product from the plastics, petroleum and pharmaceutical industries. There are different methods for treating wastewater containing the listed xenobiotics. Applied physicochemical methods are often economically ineffective and may cause other toxic products to occur. For this reason, microbiological treatment methods are preferred. We tested three different bacterial strains: Pseudomonas putida, Bradyrhizobium japonicum and Xanthobacter autotrophicus GJ10. In our studies for a period of 14 days with pre-adapted culture of Pseudomonas putida strain, we have achieved a degradation of 0.26 g/l of phenol in shaking flasks and a fed-batch process.. Over a period of 6 days, 1.2 g/l of 1,2 dibromethane was degraded using the Bradyrhizobium japonicum strain, and for 3 days Xanthobacter autotrophicus GJ10 degraded 0.9 g / l of 1,2-dichloroethane.