MOLECULAR - GENETIC IDENTIFICATION OF LACTIC ACID BACTERIA ON THE BASIS OF THE ANALYSIS OF THE NUCLEOTIDE SEQUENCES OF 16S RRNA GENE
Published: 23 May 2015
Abstract: Molecular-genetic analysis of the nucleotide sequence of the 16S rRNA gene of lactic acid bacteria isolated from the gastrointestinal tract healthy people. Identified to species two cultures bacteria of the genus Lactobacillus, which are antagonists against pathogenic and conditionally pathogenic bacteria. By results of the analysis of the nucleotide sequence of the 16S rRNA gene two strains are classified in the genus Lactobacillus. Phylogenetic analysis of the fragment of the 16S rRNA gene showed that strains Lactobacillus plantarum. №22 and Lactobacillus cellobiosus №20 form one cluster with strains of Lactobacillus farraginis and Lactobacillus pentosus. Phylogenetic analysis of the fragment of the 16S rRNA gene showed that the two strains belong to phylogenetic group Lactobacillus buchneri and Lactobacillus plantarum.
Keywords: lactobacillus, genetic identification, 16s rrna gene, primers, sequencing, phylogeny
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